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sylph-tax - incorporating taxonomy into sylph

Note

The sylph-tax repository replaces the old sylph-utils repository. sylph-tax is easier to download/install and use than sylph-utils.

Sylph's TSV outputs do not have taxonomic information. E.g., GCA_000011.fasta is a database genome which is found within your metagenome, but this contains no information about the species, genus, order, etc.

sylph-tax can turn sylph's TSV output into a taxonomic profile like Kraken or MetaPhlAn. sylph-tax does this by using custom taxonomy files to annotate sylph's output.

Taxonomy integration - available databases with taxonomy files

The following pre-built sylph databases have available taxonomic annotations. Custom taxonomies can also be incorporated.

sylph-tax identifier (used in taxprof command) Database description Clades
GTDB_r220 GTDB-r220 (April 2024) Prokaryote
GTDB_r214 GTDB-r214 (April 2023) Prokaryote
OceanDNA OceanDNA - ocean MAGs from Nishimura & Yoshizawa Prokaryote
SoilSMAG Soil MAGs (SMAG) from Ma et al. Prokaryote
FungiRefSeq-2024-07-25 Refseq fungi representative genomes collected on 2024-07-25 Eukaryote
TaraEukaryoticSMAG TARA eukaryotic SMAGs from Delmont et al. Eukaryote
IMGVR_4.1 IMG/VR 4.1 high-confidence viral OTU genomes Virus

How do I use and install sylph-tax?

See the sylph-tax install and quick start guide here or the navigation side bar.